cellular senescence β gal assay kit  (Cell Signaling Technology Inc)

Journal: bioRxiv

Article Title: Myeloid-specific TFAM deficiency drives mitochondrial DNA stress and exacerbates allergic airway inflammation

doi: 10.1101/2025.05.27.654580

Figure Lengend Snippet: Pharmacological inhibition of senescence improves asthmatic inflammation from DRA-challenged mice. (A) qRT-PCR quantification of the levels of Irf4, Ccl17, Arg1 in BMDM from WT. BMDM were treated with indicated concentrations of ABT-263 (ABT), stimulated with IL-4 and TGFβ. (B) Schematic illustration of ABT-263 (ABT, 1 mg/kg) treatment in DRA-induced mouse asthma model. Total cells and eosinophils influx in BALF were counted based on total amount of BAL cells in WT mice, analyzed by flow cytometry. (C) Total number of cells in DRA-exposed WT and TFAM KO mice. (D) Level of cytokines in BALF of ABT-263 (ABT, 1 mg/kg) treated DRA-exposed WT and TFAM KO mice by ELISA. (E) Lung SA-β-gal activity was measured from WT and TFAM KO using SA-β-gal activity kit. Graphs are plotted as mean ± SD. p-Values were obtained using one-way ANOVA followed by Tukey’s multiple comparison tests. *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001.

Article Snippet: Senescence associated-β-galactosidase (SA-β-gal) activity from cells and homogenized lung tissues [ , ] were measured quantitatively using cellular senescence activity assay kit (Cell Signaling Technology) according to the manufacturer’s protocol.

Techniques: Inhibition, Quantitative RT-PCR, Flow Cytometry, Enzyme-linked Immunosorbent Assay, Activity Assay, Comparison

Journal: Bioengineered

Article Title: The protective effects of etomidate against interleukin-1β (IL-1β)-induced oxidative stress, extracellular matrix alteration and cellular senescence in chondrocytes

doi: 10.1080/21655979.2021.2016085

Figure Lengend Snippet: Etomidate mitigates IL-1β-induced cellular senescence. Cells were incubated with IL-1β (10 ng/mL) in the presence or absence of 3 and 6 μM etomidate for 7 days. Cellular senescence was assayed with senescence-associated β- galactosidase (SA-β-Gal) staining ( ### P < 0.005 vs. vehicle group; * , **P < 0.05, 0.01 vs. IL-1β group).

Article Snippet: SA-β-Gal activity in C28/I2 chondrocytes was determined using a cellular senescence staining kit (Cell Signaling Technology, Boston, MA, USA).

Techniques: Incubation, Staining

Journal: Bioengineered

Article Title: The protective effects of etomidate against interleukin-1β (IL-1β)-induced oxidative stress, extracellular matrix alteration and cellular senescence in chondrocytes

doi: 10.1080/21655979.2021.2016085

Figure Lengend Snippet: Blockage of AMPK abolishes the protective effects of Etomidate against IL-1β- induced cellular senescence. Cells were incubated with IL-1β (10 ng/mL) in the presence or absence of 6 μM Etomidate or the AMPK inhibitor compound C (10 μM). (a). mRNA levels of PAI-1 and p16; (b). Telomerase activity; (c). SA-β-Gal staining ( ### P < 0.005 vs. vehicle group; * , **P < 0.05, 0.01 vs. IL-1β group).

Article Snippet: SA-β-Gal activity in C28/I2 chondrocytes was determined using a cellular senescence staining kit (Cell Signaling Technology, Boston, MA, USA).

Techniques: Incubation, Activity Assay, Staining